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验证数据展示

  • WB analysis of human skeletal muscle using 15814-1-AP

    WB analysis of human skeletal muscle using 15814-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15814-1-AP (MYL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat skeletal muscle using 15814-1-AP

    IHC staining of rat skeletal muscle using 15814-1-AP

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 15814-1-AP (MYL1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MYL1 antibody included
  • IHC staining of rat skeletal muscle using 15814-1-AP

    IHC staining of rat skeletal muscle using 15814-1-AP

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 15814-1-AP (MYL1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MYL1 antibody included
  • IHC staining of mouse skeletal muscle using 15814-1-AP

    IHC staining of mouse skeletal muscle using 15814-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 15814-1-AP (MYL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MYL1 antibody included
  • IHC staining of rat skeletal muscle using 15814-1-AP

    IHC staining of rat skeletal muscle using 15814-1-AP

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 15814-1-AP (MYL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MYL1 antibody included

MYL1 Polyclonal antibody

MYL1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15814-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

MLC1/MLC3, MLC1F, MLC1F/MLC3F, MLC3F, MYL1, Myosin light chain A1/A2, Myosin light chain alkali 1/2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman skeletal muscle tissue, human heart tissue, mouse heart tissue, mouse skeletal muscle tissue, mouse skin tissue
Positive IHC detected inrat skeletal muscle tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy MYL1 Ready-To-Use IHC Kit. MYL1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

15814-1-AP targets MYL1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原

CatNo: Ag8594

Product name: Recombinant human MYL1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-150 aa of BC005318

Sequence: MSFSADQIAEFKEAFLLFDRTGDSKITLSQVGDVLRALGTNPTNAEVRKVLGNPSNEELNAKKIEFEQFLPMMQAISNNKDQATYEDFVEGLRVFDKEGNGTVMGAELRHVLATLGEKMKEEEVEALMAGQEDSNGCINYEAFVKHIMSI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 myosin, light chain 1, alkali; skeletal, fast
别名 MLC1/MLC3, MLC1F, MLC1F/MLC3F, MLC3F, MYL1, Myosin light chain A1/A2, Myosin light chain alkali 1/2
计算分子量150aa,17 kDa; 194aa,22 kDa
观测分子量 14-17 kDa, 22-25 kDa
GenBank蛋白编号BC005318
基因名称 MYL1
Gene ID (NCBI) 4632
RRIDAB_2266747
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP05976
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MYL1 antibody 15814-1-APDownload protocol
IHC protocol for MYL1 antibody 15814-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Nature

SAR1B senses leucine levels to regulate mTORC1 signalling.

Authors - Jie Chen
View Article
mouseWB

Cell Prolif

KLF3 promotes the 8-cell-like transcriptional state in pluripotent stem cells.

Authors - Jing Hao
View Article