验证数据展示
经过测试的应用
| Positive WB detected in | mouse small intestine tissue, HEK-293 cells, rat small intestine |
| Positive IP detected in | HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 12 publications below |
产品信息
19514-1-AP targets MOGAT2 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, pig |
| 免疫原 |
fusion protein 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | monoacylglycerol O-acyltransferase 2 |
| 别名 | DC5, DGAT2L5, hDC5, hMGAT2, MGAT2, MOGAT2 |
| 计算分子量 | 38 kDa |
| 观测分子量 | 36-38 kDa |
| GenBank蛋白编号 | NM_025098 |
| 基因名称 | MOGAT2 |
| Gene ID (NCBI) | 80168 |
| RRID | AB_10638917 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q3SYC2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MOGAT2, also named as DC5 and DGAT2L5, belongs to the diacylglycerol acyltransferase family. MOGAT2 catalyze the reaction: Acyl-CoA + 2-acylglycerol = CoA + diacylglycerol. MOGAT2 has a preference toward monoacylglycerols containing unsaturated fatty acids. MOGAT2 plays a central role in absorption of dietary fat in the small intestine by catalyzing the resynthesis of triacylglycerol in enterocytes. And it plays a role in diet-induced obesity. The antibody can recognize all these 3 isoform of MOGAT2.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MOGAT2 antibody 19514-1-AP | Download protocol |
| IP protocol for MOGAT2 antibody 19514-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Metab Adipocyte iron levels impinge on a fat-gut crosstalk to regulate intestinal lipid absorption and mediate protection from obesity. | ||
Nat Cell Biol AIDA directly connects sympathetic innervation to adaptive thermogenesis by UCP1. | ||
Cell Metab AIDA Selectively Mediates Downregulation of Fat Synthesis Enzymes by ERAD to Retard Intestinal Fat Absorption and Prevent Obesity. | ||
Cell Res DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling. | ||
Front Immunol Fatty acid metabolism prognostic signature predicts tumor immune microenvironment and immunotherapy, and identifies tumorigenic role of MOGAT2 in lung adenocarcinoma |
