验证数据展示

WB analysis using 17564-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17564-1-AP (CD146/MCAM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 17564-1-AP
WB result of CD146/MCAM antibody (17564-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD146/MCAM transfected HepG2 cells.
IHC staining of human ovary cancer using 17564-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 17564-1-AP (CD146/MCAM antibody) at dilution of 1:40000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with CD146/MCAM antibody included
IHC staining of human ovary cancer using 17564-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 17564-1-AP (CD146/MCAM antibody) at dilution of 1:40000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with CD146/MCAM antibody included
IF Staining of mouse spleen using 17564-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD146/MCAM antibody (17564-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse spleen using 17564-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD146/MCAM antibody (17564-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP experiment of HeLa using 17564-1-AP
IP result of anti-CD146/MCAM (IP:17564-1-AP, 4ug; Detection:17564-1-AP 1:1500) with HeLa cells lysate 4000ug.
IF Staining of HUVEC using 17564-1-AP
Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using CD146/MCAM antibody (17564-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO验证

CD146/MCAM Polyclonal antibody

CD146/MCAM Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Cat No. 17564-1-AP

产品说明书

引用文献(32)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, IF/ICC, IF-P, IP, ELISA and More (2)

别名

CD146, MCAM, Melanoma-associated antigen A32, Melanoma-associated antigen MUC18, MUC18

缓冲液配方: PBS, Azide, Glycerol

PBS, Azide, Glycerol

偶联物: Unconjugated

Unconjugated

CoraLite® Plus 488

规格	价格	库存

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概述

经过测试的应用
产品信息
发表文章 (32)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	HeLa cells, A375 cells, mouse lung tissue, HUVEC cells, HepG2 cells
Positive IP detected in	HeLa cells, HepG2 cells
Positive IHC detected in	human ovary cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse spleen tissue
Positive IF/ICC detected in	HUVEC cells

Planning an IHC experiment? We recommend our IHCeasy CD146/MCAM Ready-To-Use IHC Kit. CD146/MCAM primary antibody included.

Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

应用	推荐稀释比
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20000-1:80000
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 5 publications below
WB	See 11 publications below
IHC	See 9 publications below
IF	See 13 publications below
IP	See 1 publications below

产品信息

17564-1-AP targets CD146/MCAM in WB, IHC, IF/ICC, IF-P, IP, ELISA, Cell treatment applications and shows reactivity with human, mouse, rat samples.

经测试应用	WB, IHC, IF/ICC, IF-P, IP, ELISA Application Description
文献引用应用	WB, IHC, IF, IP, Cell treatment
经测试反应性	human, mouse, rat
文献引用反应性	human, mouse, rat, rabbit
免疫原	CatNo: Ag11762 Product name: Recombinant human CD146, MCAM protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 26-350 aa of BC056418 Sequence: GEAEQPAPELVEVEVGSTALLKCGLSQSQGNLSHVDWFSVHKEKRTLIFRVRQGQGQSEPGEYEQRLSLQDRGATLALTQVTPQDERIFLCQGKRPRSQEYRIQLRVYKAPEEPNIQVNPLGIPVNSKEPEEVATCVGRNGYPIPQVIWYKNGRPLKEEKNRVHIQSSQTVESSGLYTLQSILKAQLVKEDKDAQFYCELNYRLPSGNHMKESREVTVPVFYPTEKVWLEVEPVGMLKEGDRVEIRCLADGNPPPHFSISKQNPSTREAEEETTNDNGVLVLEPARKEHSGRYECQGLDLDTMISLLSEPQELLVNYVSDVRVSP 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	melanoma cell adhesion molecule
别名	CD146, MCAM, Melanoma-associated antigen A32, Melanoma-associated antigen MUC18, MUC18
计算分子量	646 aa, 72 kDa
观测分子量	113-120 kDa
GenBank蛋白编号	BC056418
基因名称	CD146
Gene ID (NCBI)	4162
RRID	AB_2143373
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	P43121
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

CD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein of 113-130 kDa, belonging to the immunoglobulin (Ig) superfamily (PMID: 8378324; 25993332). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs) (PMID: 19356677). In addition to the membrane-anchored form of CD146, a soluble form of CD146 (sCD146, 105 kDa) has also been found in human plasma and in the supernatant of cultured human endothelial cells (PMID: 9462829; 19229070; 16374253; 14597988).

实验方案

Product Specific Protocols
IF protocol for CD146/MCAM antibody 17564-1-AP	Download protocol
IHC protocol for CD146/MCAM antibody 17564-1-AP	Download protocol
IP protocol for CD146/MCAM antibody 17564-1-AP	Download protocol
WB protocol for CD146/MCAM antibody 17564-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
mouse	IHC	Biomaterials Macropore design of tissue engineering scaffolds regulates mesenchymal stem cell differentiation fate. Authors - W Benton Swanson View Article
rat	IF	Adv Healthc Mater Extrafibrillarly Demineralized Dentin Matrix for Bone Regeneration Authors - Xiaoyi Wu View Article
human	WB	Oncogene The histone demethylase KDM3A, and its downstream target MCAM, promote Ewing Sarcoma cell migration and metastasis. Authors - M Sechler KD Validated View Article
human	IF	Cell Prolif Dental pulp stem cells-based therapy for the oviduct injury via immunomodulation and angiogenesis in vivo. Authors - Lihua Luo View Article
human		Stem Cell Res Ther Connective tissue growth factor promotes cementogenesis and cementum repair via Cx43/β-catenin axis Authors - Zuping Wu View Article
rat	WB	J Proteome Res Comparative Proteomic Analysis of Primary Schwann Cells and a Spontaneously Immortalized Schwann Cell Line RSC 96: A Comprehensive Overview with a Focus on Cell Adhesion and Migration Related Proteins. Authors - Ji Yuhua Y KD Validated View Article

验证数据展示

WB analysis using 17564-1-AP

WB analysis of HepG2 using 17564-1-AP

IHC staining of human ovary cancer using 17564-1-AP

IHC staining of human ovary cancer using 17564-1-AP

IF Staining of mouse spleen using 17564-1-AP

IF Staining of mouse spleen using 17564-1-AP

IP experiment of HeLa using 17564-1-AP

IF Staining of HUVEC using 17564-1-AP

CD146/MCAM Polyclonal antibody

CD146/MCAM Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Macropore design of tissue engineering scaffolds regulates mesenchymal stem cell differentiation fate.

Extrafibrillarly Demineralized Dentin Matrix for Bone Regeneration

The histone demethylase KDM3A, and its downstream target MCAM, promote Ewing Sarcoma cell migration and metastasis.

Dental pulp stem cells-based therapy for the oviduct injury via immunomodulation and angiogenesis in vivo.

Connective tissue growth factor promotes cementogenesis and cementum repair via Cx43/β-catenin axis

Comparative Proteomic Analysis of Primary Schwann Cells and a Spontaneously Immortalized Schwann Cell Line RSC 96: A Comprehensive Overview with a Focus on Cell Adhesion and Migration Related Proteins.