验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, rat brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
| IHC | See 1 publications below |
产品信息
20659-1-AP targets L1CAM/CD171 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | L1 cell adhesion molecule |
| 别名 | L1CAM, CAML1, CD171, HSAS, HSAS1 |
| 计算分子量 | 140 kDa |
| 观测分子量 | 200-220 kDa, 80 kDa |
| GenBank蛋白编号 | NM_000425 |
| 基因名称 | L1CAM |
| Gene ID (NCBI) | 3897 |
| RRID | AB_2878717 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P32004 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
L1CAM, also known as NCAM-L1 or CD171, is a cell adhesion molecule of the immunoglobulin superfamily. It is a 200-220 kDa transmembrane glycoprotein composed of six Ig-like domains and five fibronectin type III repeats followed by a transmembrane region and a highly conserved cytoplasmic tail (PMID: 3412448; 22796939). L1CAM is primarily expressed in the nervous system and is involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, cerebellar granule cell migration, neurite outgrowth on Schwann cells and interactions among epithelial cells of intestinal crypts (PMID: 3412448; 10767310). L1CAM is overexpressed in many human cancers and is often associated with bad prognosis (PMID: 27267927; 26111503). It has been reported that L1CAM can be proteolytically cleaved into a soluble fragment of 140 kDa and an intracellular fragment of 80-85 kDa (PMID: 30842511; 34380733). This antibody raised against 1206-1221aa of human L1CAM detects 200-220 kDa full-length L1CAM and 80-kDa cleaved fragment of L1CAM.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for L1CAM/CD171 antibody 20659-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Histopathology Frequent KRAS mutations in oncocytic papillary renal neoplasm with inverted nuclei. | ||
Diagnostics (Basel) Circulatory Astrocyte and Neuronal EVs as Potential Biomarkers of Neurological Dysfunction in HIV-Infected Subjects and Alcohol/Tobacco Users. | ||
Anal Chem Nanosensor-Driven Detection of Neuron-Derived Exosomal Aβ42 with Graphene Electrolyte-Gated Transistor for Alzheimer's Disease Diagnosis | ||
Res Sq Curcumin enhances elvitegravir concentration and alleviates oxidative stress and inflammatory response | ||
Sci Rep Curcumin enhances elvitegravir concentration and alleviates oxidative stress and inflammatory response |
