验证数据展示
经过测试的应用
| Positive WB detected in | HCT 116 cells, HeLa cells, pig skin tissue, A431 cells, HepG2 cells, COLO 320 cells, K-562 cells, 4T1 cells |
| Positive IHC detected in | human kidney tissue, human breast cancer tissue, human colon cancer tissue, human liver tissue, human lung cancer tissue, human renal cell carcinoma tissue, human tonsillitis tissue, mouse liver tissue, rat liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse testis tissue |
| Positive IF/ICC detected in | A549 cells, HUVEC cells, HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunohistochemistry (IHC) | IHC : 1:600-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66187-1-Ig targets Cytokeratin 18 in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| 经测试应用 | WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat, pig |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag1260 Product name: Recombinant human KRT18 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 127-430 aa of BC000180 Sequence: SHYFKIIEDLRAQIFANTVDNARIVLQIDNARLAADDFRVKYETELAMRQSVENDIHGLRKVIDDTNITRLQLETEIEALKEELLFMKKNHEEEVKGLQAQIASSGLTVEVDAPKSQDLAKIMADIRAQYDELARKNREELDKYWSQQIEESTTVVTTQSAEVGAAETTLTELRRTVQSLEIDLDSMRNLKASLENSLREVEARYALQMEQLNGILLHLESELAQTRAEGQRQAQEYEALLNIKVKLEAEIATYRRLLEDGEDFNLGDALDSSNSMQTIQKTTTRRIVDGKVVSETNDTKVLRH 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | keratin 18 |
| 别名 | KRT18, 1G11C4, Cell proliferation-inducing gene 46 protein, CK 18, CK18 |
| 计算分子量 | 48 kDa |
| 观测分子量 | 48 kDa |
| GenBank蛋白编号 | BC000180 |
| 基因名称 | Cytokeratin 18 |
| Gene ID (NCBI) | 3875 |
| RRID | AB_2881582 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P05783 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT18, also named as CYK18, PIG46 and K18, is the most commonly found members of the intermediate filament gene family. KRT18 is a type I keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Cytokeratin 18 antibody 66187-1-Ig | Download protocol |
| IHC protocol for Cytokeratin 18 antibody 66187-1-Ig | Download protocol |
| IF protocol for Cytokeratin 18 antibody 66187-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Blebbisomes are large, organelle-rich extracellular vesicles with cell-like properties | ||
Redox Biol ACSL4 deficiency confers protection against ferroptosis-mediated acute kidney injury. | ||
J Pathol Histone demethylase PHF8 drives neuroendocrine prostate cancer progression by epigenetically upregulating FOXA2. | ||
Hum Reprod T-cadherin inhibits invasion and migration of endometrial stromal cells in endometriosis. | ||
Stem Cell Res Ther Expansion of mouse castration-resistant intermediate prostate stem cells in vitro | ||
Commun Biol Single-cell analysis supports a luminal-neuroendocrine transdifferentiation in human prostate cancer. |
