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验证数据展示

  • WB analysis using 60074-1-Ig (same clone as 60074-1-PBS)

    WB analysis using 60074-1-Ig (same clone as 60074-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60074-1-Ig (IFITM1-Specific antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60074-1-PBS in a different storage buffer formulation.

  • WB analysis of TF-1 using 60074-1-Ig (same clone as 60074-1-PBS)

    WB analysis of TF-1 using 60074-1-Ig (same clone as 60074-1-PBS)

    TF-1 cells were subjected to SDS PAGE followed by western blot with 60074-1-Ig (IFITM1-Specific antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60074-1-PBS in a different storage buffer formulation.

  • IP experiment of HepG2 using 60074-1-Ig (same clone as 60074-1-PBS)

    IP experiment of HepG2 using 60074-1-Ig (same clone as 60074-1-PBS)

    IP result of anti-IFITM1-Specific (IP:60074-1-Ig, 4ug; Detection:60074-1-Ig 1:500) with HepG2 cells lysate 3600ug. This data was developed using the same antibody clone with 60074-1-PBS in a different storage buffer formulation.

  • IHC staining of human lymphoma using 60074-1-Ig (same clone as 60074-1-PBS)

    IHC staining of human lymphoma using 60074-1-Ig (same clone as 60074-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60074-1-Ig (IFITM1-Specific Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60074-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IFITM1 antibody included
  • IHC staining of human lymphoma using 60074-1-Ig (same clone as 60074-1-PBS)

    IHC staining of human lymphoma using 60074-1-Ig (same clone as 60074-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60074-1-Ig (IFITM1-Specific Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60074-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IFITM1 antibody included
  • IF Staining of Jurkat using 60074-1-Ig (same clone as 60074-1-PBS)

    IF Staining of Jurkat using 60074-1-Ig (same clone as 60074-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using IFITM1-Specific antibody (60074-1-Ig, Clone: 5B5E2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 60074-1-PBS in a different storage buffer formulation.

  • IF Staining of K-562 using 60074-1-Ig (same clone as 60074-1-PBS)

    IF Staining of K-562 using 60074-1-Ig (same clone as 60074-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using IFITM1-Specific antibody (60074-1-Ig, Clone: 5B5E2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 60074-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

IFITM1-Specific Monoclonal antibody, PBS Only

IFITM1-Specific Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 60074-1-PBS

产品说明书

CloneNo. 5B5E2

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, IHC, IF/ICC, IP, ELISA

别名

IFITM1, 5B5E2, 9 27, 927, CD225

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy IFITM1 Ready-To-Use IHC Kit. IFITM1 primary antibody included.

产品信息

60074-1-PBS targets IFITM1-Specific in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag2320

Product name: Recombinant human IFITM1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-125 aa of BC000897

Sequence: MHKEEHEVAVLGAPPSTILPRSTVINIHSETSVPDHVVWSLFNTLFLNWCCLGFIAFAYSVKSRDRKMVGDVTGAQAYASTAKCLNIWALILGILMTIGFILLLVFGSVTVYHIMLQIIQEKRGY

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 interferon induced transmembrane protein 1 (9-27)
别名 IFITM1, 5B5E2, 9 27, 927, CD225
计算分子量 14 kDa
观测分子量 14-17 kDa
GenBank蛋白编号BC000897
基因名称 IFITM1
Gene ID (NCBI) 8519
RRIDAB_2233405
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP13164
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IFITM1(interferon induced transmembrane protein), also named DSPA2a and interferon-induced protein 17 (IFI17), belongs to the CD225 family. It has two transmembrane domain and serves as an IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, influenza A H1N1 virus, West Nile virus (WNV), and dengue virus , by inhibiting the early steps of replication. IFITM proteins are recently identified as viral restriction factors that inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. Also they serve as important components of the innate immune system to restrict HIV-1 infection. 60074-1-Ig is a mouse monoclonal antibody which specifically recognizing IFITM1 but not IFITM2 or IFITM3.