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验证数据展示

  • WB analysis using 66013-1-Ig (same clone as 66013-1-PBS)

    WB analysis using 66013-1-Ig (same clone as 66013-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66013-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66013-1-Ig (same clone as 66013-1-PBS)

    IHC staining of human tonsillitis using 66013-1-Ig (same clone as 66013-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66013-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HLA-A antibody included
  • IHC staining of human tonsillitis using 66013-1-Ig (same clone as 66013-1-PBS)

    IHC staining of human tonsillitis using 66013-1-Ig (same clone as 66013-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66013-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HLA-A antibody included
  • IP experiment of HepG2 using 66013-1-Ig (same clone as 66013-1-PBS)

    IP experiment of HepG2 using 66013-1-Ig (same clone as 66013-1-PBS)

    IP result of anti-HLA class I ABC (IP:66013-1-Ig, 4ug; Detection:66013-1-Ig 1:4000) with HepG2 cells lysate 2000 ug. This data was developed using the same antibody clone with 66013-1-PBS in a different storage buffer formulation.

  • FC experiment of Raji using 66013-1-Ig (same clone as 66013-1-PBS)

    FC experiment of Raji using 66013-1-Ig (same clone as 66013-1-PBS)

    1X10^6 Raji cells were surface stained with 0.4 ug Anti-Human HLA class I ABC (66013-1-Ig, Clone:5C5B7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were not fixed. This data was developed using the same antibody clone with 66013-1-PBS in a different storage buffer formulation.

  • IF Staining of Raji using 66013-1-Ig (same clone as 66013-1-PBS)

    IF Staining of Raji using 66013-1-Ig (same clone as 66013-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using HLA class I ABC antibody (66013-1-Ig, Clone: 5C5B7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66013-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

HLA class I ABC Monoclonal antibody, PBS Only

HLA class I ABC Monoclonal Antibody for WB, IHC, IF/ICC, FC, IP, Indirect ELISA
Cat No. 66013-1-PBS

产品说明书

CloneNo. 5C5B7

宿主/亚型

Mouse / IgG2b

种属反应性

human, pig

应用

WB, IHC, IF/ICC, FC, IP, Indirect ELISA

别名

HLA-A, 5C5B7, HLA A, HLA class I, HLA class I (HLA-A)

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy HLA-A Ready-To-Use IHC Kit. HLA-A primary antibody included.

产品信息

66013-1-PBS targets HLA class I ABC in WB, IHC, IF/ICC, FC, IP, Indirect ELISA applications and shows reactivity with human, pig samples.

经测试应用 WB, IHC, IF/ICC, FC, IP, Indirect ELISA Application Description
经测试反应性 human, pig
免疫原

CatNo: Ag7488

Product name: Recombinant human HLA class I (HLA-A) protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 28-303 aa of BC003069

Sequence: SMRYFFTSVSRPGRGEPRFIAVGYVDDTQFVRFDSDAASQKMEPRAPWIEQEGPEYWDQETRNMKAHSQTDRANLGTLRGYYNQSEDGSHTIQIMYGCDVGPDGRFLRGYRQDAYDGKDYIALNEDLRSWTAADMAAQITKRKWEAVHAAEQRRVYLEGRCVDGLRRYLENGKETLQRTDPPKTHMTHHPISDHEATLRCWALGFYPAEITLTWQRDGEDQTQDTELVETRPAGDGTFQKWAAVVVPSGEEQRYTCHVQHEGLPKPLTLRWELSSQ

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 major histocompatibility complex, class I, A
别名 HLA-A, 5C5B7, HLA A, HLA class I, HLA class I (HLA-A)
计算分子量 41 kDa
观测分子量 40-44 kDa
GenBank蛋白编号BC003069
基因名称 HLA-A
Gene ID (NCBI) 3105
RRIDAB_11042593
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP04439
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system. (PMID: 667938; 3375250)

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