验证数据展示

WB analysis of HeLa cells using 10758-1-AP
WB result of HADHA antibody (10758-1-AP, 1:4000) with si-control and si-HADHA transfected HeLa cells.
IF Staining of HeLa cells using 10758-1-AP
IF result of HADHA antibody (10758-1-AP, 1:50) with si-control and si-HADHA transfected HeLa cells.
WB analysis using 10758-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10758-1-AP (HADHA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 10758-1-AP
IP result of anti-HADHA (IP:10758-1-AP, 4ug; Detection:10758-1-AP 1:10000) with HeLa cells lysate 1080 ug.
IHC staining of human heart using 10758-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10758-1-AP (HADHA antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with HADHA antibody included
IHC staining of human heart using 10758-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10758-1-AP (HADHA antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with HADHA antibody included
IHC staining of human colon using 10758-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10758-1-AP (HADHA antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with HADHA antibody included
IF Staining of HeLa using 10758-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HADHA antibody (10758-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

Featured Product
KD/KO验证

HADHA Polyclonal antibody

HADHA Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10758-1-AP

产品说明书

引用文献(30)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (3)

应用

WB, IHC, IF/ICC, IP, ELISA and More (2)

别名

HADH, GBP, ECHA, EC:2.3.1.-, 78 kDa gastrin-binding protein

缓冲液配方: PBS and Azide

PBS and Azide

偶联物: Unconjugated

Unconjugated

规格	价格	库存

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概述

经过测试的应用
产品信息
发表文章 (30)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	Jurkat cells, HEK-293 cells, HeLa cells, mouse liver tissue, rat liver tissue, NIH/3T3 cells, mouse kidney tissue, rat kidney tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	human heart tissue, human colon tissue, human colon cancer tissue, human ovary tissue, mouse heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells, HepG2 cells

Planning an IHC experiment? We recommend our IHCeasy HADHA Ready-To-Use IHC Kit. HADHA primary antibody included.

应用	推荐稀释比
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WB	See 28 publications below
IHC	See 2 publications below
IF	See 2 publications below
IP	See 2 publications below
CoIP	See 1 publications below

产品信息

10758-1-AP targets HADHA in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用	WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用	WB, IHC, IF, IP, CoIP
经测试反应性	human, mouse, rat
文献引用反应性	human, mouse, rat, pig, monkey, zebrafish
免疫原	CatNo: Ag1211 Product name: Recombinant human HADHA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 339-763 aa of BC009235 Sequence: LMGLYHGQVLCKKNKFGAPQKDVKHLAILGAGLMGAGIAQVSVDKGLKTILKDATLTALDRGQQQVFKGLNDKVKKKALTSFERDSIFSNLTGQLDYQGFEKADMVIEAVFEDLSLKHRVLKEVEAVIPDHCIFASNTSALPISEIAAVSKRPEKVIGMHYFSPVDKMQLLEIITTEKTSKDTSASAVAVGLKQGKVIIVVKDGPGFYTTRCLAPMMSEVIRILQEGVDPKKLDSLTTSFGFPVGAATLVDEVGVDVAKHVAEDLGKVFGERFGGGNPELLTQMVSKGFLGRKSGKGFYIYQEGVKRKDLNSDMDSILASLKLPPKSEVSSDEDIQFRLVTRFVNEAVMCLQEGILATPAEGDIGAVFGLGFPPCLGGPFRFVDLYGAQKIVDRLKKYEAAYGKQFTPCQLLADHANSPNKKFYQ 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), alpha subunit
别名	HADH, GBP, ECHA, EC:2.3.1.-, 78 kDa gastrin-binding protein
计算分子量	83 kDa
观测分子量	70-79 kDa
GenBank蛋白编号	BC009235
基因名称	HADHA
Gene ID (NCBI)	3030
RRID	AB_2115593
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	P40939
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

HADHA(Trifunctional enzyme subunit alpha, mitochondrial) is also named as HADH,78 kDa gastrin-binding protein.It belongs to the enoyl-CoA hydratase/isomerase family in the N-terminal section and the 3-hydroxyacyl-CoA dehydrogenase family in the central section.It harbors the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities.Defects in HADHA are a cause of trifunctional protein deficiency (TFP deficiency) and long-chain 3-hydroxyl-CoA dehydrogenase deficiency (LCHAD deficiency) and maternal acute fatty liver of pregnancy (AFLP).

实验方案

Product Specific Protocols
WB protocol for HADHA antibody 10758-1-AP	Download protocol
IHC protocol for HADHA antibody 10758-1-AP	Download protocol
IF protocol for HADHA antibody 10758-1-AP	Download protocol
IP protocol for HADHA antibody 10758-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	WB	Cell Mitochondrial Sirtuin Network Reveals Dynamic SIRT3-Dependent Deacetylation in Response to Membrane Depolarization. Authors - Wen Yang View Article
mouse	WB	Nat Commun Moderate-intensity interval exercise exacerbates cardiac lipotoxicity in high-fat, high-calories diet-fed mice Authors - Jing Geng View Article
human	WB	Cell Metab DNAJC19, a mitochondrial cochaperone associated with cardiomyopathy, forms a complex with prohibitins to regulate cardiolipin remodeling. Authors - Ricarda Richter-Dennerlein View Article
pig	WB	Sci Total Environ Protein lysine acetylation played an important role in NH3-induced AEC2 damage and pulmonary fibrosis in piglets Authors - Xiaotong Wang View Article
mouse	WB	Cell Rep Feedback repression of PPARα signaling by Let-7 microRNA. Authors - Tomoki Yagai View Article
mouse	WB	Oxid Med Cell Longev Mild Hypothermia Attenuates Hepatic Ischemia-Reperfusion Injury through Regulating the JAK2/STAT3-CPT1a-Dependent Fatty Acid β-Oxidation. Authors - Wei Wang View Article

验证数据展示

WB analysis of HeLa cells using 10758-1-AP

IF Staining of HeLa cells using 10758-1-AP

WB analysis using 10758-1-AP

IP experiment of HeLa using 10758-1-AP

IHC staining of human heart using 10758-1-AP

IHC staining of human heart using 10758-1-AP

IHC staining of human colon using 10758-1-AP

IF Staining of HeLa using 10758-1-AP

HADHA Polyclonal antibody

HADHA Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Mitochondrial Sirtuin Network Reveals Dynamic SIRT3-Dependent Deacetylation in Response to Membrane Depolarization.

Moderate-intensity interval exercise exacerbates cardiac lipotoxicity in high-fat, high-calories diet-fed mice

DNAJC19, a mitochondrial cochaperone associated with cardiomyopathy, forms a complex with prohibitins to regulate cardiolipin remodeling.

Protein lysine acetylation played an important role in NH3-induced AEC2 damage and pulmonary fibrosis in piglets

Feedback repression of PPARα signaling by Let-7 microRNA.

Mild Hypothermia Attenuates Hepatic Ischemia-Reperfusion Injury through Regulating the JAK2/STAT3-CPT1a-Dependent Fatty Acid β-Oxidation.