验证数据展示

WB analysis using 10727-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10727-1-AP (Ferritin light chain antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis using 10727-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10727-1-AP (Ferritin light chain antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 10727-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10727-1-AP (Ferritin light chain antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse liver using 10727-1-AP
IP result of anti-Ferritin light chain (IP:10727-1-AP, 4ug; Detection:10727-1-AP 1:1000) with mouse liver tissue lysate 3040 ug.
IHC staining of human liver using 10727-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10727-1-AP (Ferritin light chain antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with FTL antibody included
IHC staining of human liver using 10727-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10727-1-AP (Ferritin light chain antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with FTL antibody included
IF Staining of A549 using 10727-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using Ferritin light chain antibody (10727-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Featured Product
KD/KO验证

Ferritin light chain Polyclonal antibody

Ferritin light chain Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10727-1-AP

产品说明书

引用文献(202)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (2)

应用

WB, IHC, IF/ICC, IP, ELISA and More (2)

别名

Ferritin, FTL, Ferritin L subunit

缓冲液配方: PBS and Azide

PBS and Azide

偶联物: Unconjugated

Unconjugated

CoraLite® Plus 488

规格	价格	库存

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概述

经过测试的应用
产品信息
发表文章 (202)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	mouse liver tissue, human placenta tissue, mouse ovary tissue, mouse testis tissue, mouse spleen tissue, rat liver tissue
Positive IP detected in	mouse liver tissue
Positive IHC detected in	human liver tissue, human liver cancer tissue, mouse spleen tissue, human spleen tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A549 cells

This antibody is not recommended for WB detection of brain sample.

Planning an IHC experiment? We recommend our IHCeasy FTL Ready-To-Use IHC Kit. FTL primary antibody included.

Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

应用	推荐稀释比
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 6 publications below
WB	See 169 publications below
IHC	See 32 publications below
IF	See 25 publications below
IP	See 1 publications below
CoIP	See 1 publications below

产品信息

10727-1-AP targets Ferritin light chain in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用	WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用	WB, IHC, IF, IP, CoIP
经测试反应性	human, mouse, rat
文献引用反应性	human, mouse, rat, monkey, duck
免疫原	CatNo: Ag1149 Product name: Recombinant human FTL protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-175 aa of BC008439 Sequence: MSSQIRQNYSTDVEAAVNSLVNLYLQASYTYLSLGFYFDRDDVALEGVSHFFRELAEEKREGYERLLKMQNQRGGRALFQDIKKPAEDEWGKTPDAMKAAMALEKKLNQALLDLHALGSARTDPHLCDFLETHFLDEEVKLIKKMGDHLTNLHRLGGPEAGLGEYLFERLTLKHD 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	ferritin, light polypeptide
别名	Ferritin, FTL, Ferritin L subunit
计算分子量	19 kDa
观测分子量	18-20 kDa
GenBank蛋白编号	BC008439
基因名称	Ferritin light chain
Gene ID (NCBI)	2512
RRID	AB_2278673
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	P02792
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

FTL belongs to the ferritin family. It stores iron in a soluble, non-toxic, readily available form. FTL is important for iron homeostasis. It plays a role in delivery of iron to cells. FTL mediates iron uptake in capsule cells of the developing kidney. Mutation of FTL will cause hereditary hyperferritinemia-cataract syndrome (HHCS) or neurodegeneration with brain iron accumulation type 3 (NBIA3). Ferritin light polypeptide (FTL) and ferritin heavy polypeptide (FTH1) were the main constituents the striatum and cerebellar cortex revealed.

实验方案

Product Specific Protocols
WB protocol for Ferritin light chain antibody 10727-1-AP	Download protocol
IHC protocol for Ferritin light chain antibody 10727-1-AP	Download protocol
IF protocol for Ferritin light chain antibody 10727-1-AP	Download protocol
IP protocol for Ferritin light chain antibody 10727-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human,mouse	WB	Cell Metab Lactate modulates iron metabolism by binding soluble adenylyl cyclase Authors - Wei Liu View Article
mouse	WB,IHC	Mil Med Res Caveolin-1 is critical for hepatic iron storage capacity in the development of nonalcoholic fatty liver disease Authors - Guang-Hui Deng View Article
human	WB	Nat Cell Biol Selective VPS34 inhibitor blocks autophagy and uncovers a role for NCOA4 in ferritin degradation and iron homeostasis in vivo. Authors - William E Dowdle KD Validated View Article
mouse	WB,IHC	ACS Nano Ferroptosis Is Involved in Sex-Specific Small Intestinal Toxicity in the Offspring of Adult Mice Exposed to Polystyrene Nanoplastics during Pregnancy Authors - Juan Tang View Article
mouse	IF	Nat Commun Targeting ferroptosis by poly(acrylic) acid coated Mn3O4 nanoparticles alleviates acute liver injury Authors - Xinyi Shan View Article
human	WB	Adv Sci (Weinh) PHOSPHO1 Suppresses Ferroptosis in Retinal Pigment Epithelial Cells by Reducing the Levels of Phosphatidylethanolamine Molecular Species Authors - Zhiyang Chen View Article

验证数据展示

WB analysis using 10727-1-AP

WB analysis using 10727-1-AP

WB analysis using 10727-1-AP

IP experiment of mouse liver using 10727-1-AP

IHC staining of human liver using 10727-1-AP

IHC staining of human liver using 10727-1-AP

IF Staining of A549 using 10727-1-AP

Ferritin light chain Polyclonal antibody

Ferritin light chain Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

经过测试的应用

This antibody is not recommended for WB detection of brain sample.

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Lactate modulates iron metabolism by binding soluble adenylyl cyclase

Caveolin-1 is critical for hepatic iron storage capacity in the development of nonalcoholic fatty liver disease

Selective VPS34 inhibitor blocks autophagy and uncovers a role for NCOA4 in ferritin degradation and iron homeostasis in vivo.

Ferroptosis Is Involved in Sex-Specific Small Intestinal Toxicity in the Offspring of Adult Mice Exposed to Polystyrene Nanoplastics during Pregnancy

Targeting ferroptosis by poly(acrylic) acid coated Mn3O4 nanoparticles alleviates acute liver injury

PHOSPHO1 Suppresses Ferroptosis in Retinal Pigment Epithelial Cells by Reducing the Levels of Phosphatidylethanolamine Molecular Species