验证数据展示

WB analysis using 14384-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14384-1-AP (Fascin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
IHC staining of human tonsillitis using 14384-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14384-1-AP (Fascin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with Fascin antibody included
IHC staining of human tonsillitis using 14384-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14384-1-AP (Fascin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with Fascin antibody included
WB analysis of HeLa using 14384-1-AP
WB result of Fascin antibody (14384-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Fascin transfected HeLa cells.
IF Staining of mouse brain using 14384-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 14384-1-AP (Fascin antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of SH-SY5Y using 14384-1-AP
1X10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Anti-Human Fascin (14384-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.
WB analysis using 14384-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14384-1-AP (Fascin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
IF Staining of U-87 MG using 14384-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed U-87 MG cells using Fascin antibody (14384-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Featured Product
KD/KO验证

Fascin Polyclonal antibody

Fascin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Cat No. 14384-1-AP

产品说明书

引用文献(13)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA and More (1)

别名

FSCN1, 55 kDa actin bundling protein, 55 kDa actin-bundling protein, FAN1, Fascin 1,Fascin

缓冲液配方: PBS and Azide

PBS and Azide

偶联物: Unconjugated

Unconjugated

规格	价格	库存

点击咨询售前客服

点击咨询售后客服

概述

经过测试的应用
产品信息
发表文章 (13)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	HeLa cells, mouse thymus tissue, K-562 cells, NIH/3T3 cells, SH-SY5Y cells, SK-N-SH cells
Positive IHC detected in	human tonsillitis tissue, human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse brain tissue
Positive IF/ICC detected in	U-87 MG cells
Positive FC (Intra) detected in	SH-SY5Y cells

Planning an IHC experiment? We recommend our IHCeasy Fascin Ready-To-Use IHC Kit. Fascin primary antibody included.

应用	推荐稀释比
Western Blot (WB)	WB : 1:2000-1:8000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 3 publications below
WB	See 10 publications below
IHC	See 2 publications below
IF	See 3 publications below

产品信息

14384-1-AP targets Fascin in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用	WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA Application Description
文献引用应用	WB, IHC, IF
经测试反应性	human, mouse
文献引用反应性	human, mouse, rat
免疫原	CatNo: Ag5816 Product name: Recombinant human Fascin protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-187 aa of BC000521 Sequence: MTANGTAEAVQIQFGLINCGNKYLTAEAFGFKVNASASSLKKKQIWTLEQPPDEAGSAAVCLRSHLGRYLAADKDGNVTCEREVPGPDCRFLIVAHDDGRWSLQSEAHRRYFGGTEDRLSCFAQTVSPAEKWSVHIAMHPQVNIYSVTRKRYAHLSARPADEIAVDRDVPWGVDSLITLAFQDQRYS 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	fascin homolog 1, actin-bundling protein (Strongylocentrotus purpuratus)
别名	FSCN1, 55 kDa actin bundling protein, 55 kDa actin-bundling protein, FAN1, Fascin 1,Fascin
计算分子量	55 kDa
观测分子量	50-55 kDa
GenBank蛋白编号	BC000521
基因名称	Fascin
Gene ID (NCBI)	6624
ENSEMBL Gene ID	ENSG00000075618
RRID	AB_2106381
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	Q16658
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

Fascin is a highly conserved and ubiquitous actin cross-linking protein that has a major function in cell motility and adhesion. Fascin localizes to a number of highly dynamic cellular structures that require strong mechanical support which include stress fibers and cellular protrusions such as microvilli, microspikes and lamellipodia. Fascin is up-regulated in many human carcinomas. It's a marker for Reed-Sternberg cells in Hodgkin's disease; it is also a highly selective marker for dendritic cells of lymphoid tissues.

实验方案

Product Specific Protocols
WB protocol for Fascin antibody 14384-1-AP	Download protocol
IHC protocol for Fascin antibody 14384-1-AP	Download protocol
IF protocol for Fascin antibody 14384-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	WB	Clin Transl Med Parkin regulates IGF2BP3 through ubiquitination in the tumourigenesis of cervical cancer Authors - Xin Sun View Article
mouse	WB,IF	Ecotoxicol Environ Saf Prenatal Perfluorooctanoic Acid (PFOA) exposure causes reproductive toxicity by disrupting the formation of transzonal projections (TZPs) and down-regulating Wnt4/β-catenin signaling pathway in progeny Authors - Hua Du View Article
human	WB	Aging (Albany NY) Exosomal lncRNA LINC01711 facilitates metastasis of esophageal squamous cell carcinoma via the miR-326/FSCN1 axis. Authors - Mei-Ling Xu View Article
human	WB	Int J Biol Macromol Recombinant porcine NK-lysin inhibits the invasion of hepatocellular carcinoma cells in vitro. Authors - Ajab Khan View Article
human	WB	Exp Cell Res Downregulation of fascin in the first trimester placental villi is associated with early recurrent miscarriage. Authors - Jones Lamptey KD Validated View Article
human	WB	J Exp Clin Cancer Res The role of microRNA-133b and its target gene FSCN1 in gastric cancer. Authors - Lihua Guo View Article

验证数据展示

WB analysis using 14384-1-AP

IHC staining of human tonsillitis using 14384-1-AP

IHC staining of human tonsillitis using 14384-1-AP

WB analysis of HeLa using 14384-1-AP

IF Staining of mouse brain using 14384-1-AP

FC experiment of SH-SY5Y using 14384-1-AP

WB analysis using 14384-1-AP

IF Staining of U-87 MG using 14384-1-AP

Fascin Polyclonal antibody

Fascin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Parkin regulates IGF2BP3 through ubiquitination in the tumourigenesis of cervical cancer

Prenatal Perfluorooctanoic Acid (PFOA) exposure causes reproductive toxicity by disrupting the formation of transzonal projections (TZPs) and down-regulating Wnt4/β-catenin signaling pathway in progeny

Exosomal lncRNA LINC01711 facilitates metastasis of esophageal squamous cell carcinoma via the miR-326/FSCN1 axis.

Recombinant porcine NK-lysin inhibits the invasion of hepatocellular carcinoma cells in vitro.

Downregulation of fascin in the first trimester placental villi is associated with early recurrent miscarriage.

The role of microRNA-133b and its target gene FSCN1 in gastric cancer.