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验证数据展示

  • WB analysis of HEK-293 using 22173-1-AP

    WB analysis of HEK-293 using 22173-1-AP

    WB result of FOXP3 antibody (22173-1-AP, 1:500) with si-Control and si-FOXP3 transfected HEK293 cells.

  • WB analysis of HEK-293 using 22173-1-AP

    WB analysis of HEK-293 using 22173-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 22173-1-AP (FOXP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Featured Product
  • KD/KO验证

FOXP3 Polyclonal antibody

FOXP3 Polyclonal Antibody for WB, ELISA
Cat No. 22173-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, ELISA and More (1)

别名

AIID, DIETER, forkhead box P3, Forkhead box protein P3, FOXP3, IPEX, JM2, PIDX, Scurfin, XPID

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, HEK-293
Planning an IHC experiment? We recommend our IHCeasy FOXP3 Ready-To-Use IHC Kit. FOXP3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22173-1-AP targets FOXP3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原

CatNo: Ag17827

Product name: Recombinant human FOXP3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 230-431 aa of BC113401

Sequence: AQCLLQREMVQSLEQQLVLEKEKLSAMQAHLAGKMALTKASSVASSDKGSCCIVAAGSQGPVVPAWSGPREAPDSLFAVRRHLWGSHGNSTFPEFLHNMDYFKFHNMRPPFTYATLIRWAILEAPEKQRTLNEIYHWFTRMFAFFRNHPATWKNAIRHNLSLHKCFVRVESEKGAVWTVDELEFRKKRSQRPSRCSNPTPGP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 forkhead box P3
别名 AIID, DIETER, forkhead box P3, Forkhead box protein P3, FOXP3, IPEX, JM2, PIDX, Scurfin, XPID
计算分子量 431 aa, 47 kDa
观测分子量 47-50 kDa
GenBank蛋白编号BC113401
基因名称 FOXP3
Gene ID (NCBI) 50943
RRIDAB_2879017
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BZS1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FOXP3, also named as IPEX, JM2 and Scurfin, is a transcription factor. FOXP3 plays a critical role in the control of immune response. Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) which also known as X-linked autoimmunity-immunodeficiency syndrome. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human FOXP3.

实验方案

Product Specific Protocols
WB protocol for FOXP3 antibody 22173-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Exp Ther Med

Poly(ADP-ribose) polymerase-1 inhibitor ameliorates dextran sulfate sodium-induced colitis in mice by regulating the balance of Th17/Treg cells and inhibiting the NF-κB signaling pathway.

Authors - Shuai Peng
View Article
humanIHC

Oral Dis

Influence of PD-1/PD-L1 on immune micro-environment in oral leukoplakia and oral squamous cell carcinomas

Authors - Qiong Wang
View Article