验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HepG2 cells, human placenta tissue, Hela cells, Jurkat cells, NIH/3T3 cells, C6 cells, mouse liver tissue, rat liver tissue, L02 cells |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human placenta tissue, human normal colon, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11375-1-AP targets FH in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, caenorhabditis elegans |
| 免疫原 |
CatNo: Ag1934 Product name: Recombinant human FH protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 204-510 aa of BC003108 Sequence: HEVLLPGLQKLHDALDAKSKEFAQIIKIGRTHTQDAVPLTLGQEFSGYVQQVKYAMTRIKAAMPRIYELAAGGTAVGTGLNTRIGFAEKVAAKVAALTGLPFVTAPNKFEALAAHDALVELSGAMNTTACSLMKIANDIRFLGSGPRSGLGELILPENEPGSSIMPGKVNPTQCEAMTMVAAQVMGNHVAVTVGGSNGHFELNVFKPMMIKNVLHSARLLGDASVSFTENCVVGIQANTERINKLMNESLMLVTALNPHIGYDKAAKIAKTAHKNGSTLKETAIELGYLTAEQFDEWVKPKDMLGPK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | fumarate hydratase |
| 别名 | HsFH, Fumarase, EC:4.2.1.2 |
| 计算分子量 | 510 aa, 55 kDa |
| 观测分子量 | 45-50 kDa |
| GenBank蛋白编号 | BC003108 |
| 基因名称 | FH |
| Gene ID (NCBI) | 2271 |
| RRID | AB_763171 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P07954 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Fumarate hydratase, or fumarase, is an enzymatic component of the tricarboxylic acid cycle, catalyzing the conversion of fumarate to malate. It belongs to the class-II fumarase/aspartase family. This protein has 2 isoforms produced by alternative initiation.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for FH antibody 11375-1-AP | Download protocol |
| IHC protocol for FH antibody 11375-1-AP | Download protocol |
| IP protocol for FH antibody 11375-1-AP | Download protocol |
| WB protocol for FH antibody 11375-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Lin28/let-7 axis regulates aerobic glycolysis and cancer progression via PDK1. | ||
Aging Dis Dietary Salt Disrupts Tricarboxylic Acid Cycle and Induces Tau Hyperphosphorylation and Synapse Dysfunction during Aging | ||
Theranostics EBV(LMP1)-induced metabolic reprogramming inhibits necroptosis through the hypermethylation of the RIP3 promoter. | ||
Invest Ophthalmol Vis Sci Proteomics of uveal melanomas suggests HSP-27 as a possible surrogate marker of chromosome 3 loss. | ||
Cell Biochem Biophys sPLA2-IB and PLA2R Mediate Aberrant Glucose Metabolism in Podocytes via Hyperactivation of the mTOR/HIF-1α Pathway |
