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验证数据展示

  • WB analysis using 14768-1-AP

    WB analysis using 14768-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14768-1-AP (FEN1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse small intestine using 14768-1-AP

    IHC staining of mouse small intestine using 14768-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 14768-1-AP (FEN1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FEN1 antibody included
  • IHC staining of human lung cancer using 14768-1-AP

    IHC staining of human lung cancer using 14768-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14768-1-AP (FEN1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FEN1 antibody included
  • IHC staining of mouse ovary using 14768-1-AP

    IHC staining of mouse ovary using 14768-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 14768-1-AP (FEN1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FEN1 antibody included
  • IP experiment of NIH/3T3 using 14768-1-AP

    IP experiment of NIH/3T3 using 14768-1-AP

    IP result of anti-FEN1 (IP:14768-1-AP, 4ug; Detection:14768-1-AP 1:500) with NIH/3T3 cells lysate 1200ug.

  • IHC staining of human colon cancer using 14768-1-AP

    IHC staining of human colon cancer using 14768-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14768-1-AP (FEN1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FEN1 antibody included
  • IF Staining of NIH/3T3 using 14768-1-AP

    IF Staining of NIH/3T3 using 14768-1-AP

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using FEN1 antibody (14768-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

FEN1 Polyclonal antibody

FEN1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 14768-1-AP

产品说明书

引用文献(13)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

别名

hFEN-1, hFEN 1, Flap structure-specific endonuclease 1, FEN-1, FEN 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, NIH/3T3 cells
Positive IP detected inNIH/3T3 cells
Positive IHC detected inhuman colon cancer tissue, human lung cancer tissue, mouse ovary tissue, mouse small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy FEN1 Ready-To-Use IHC Kit. FEN1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14768-1-AP targets FEN1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP
经测试反应性 human, mouse
文献引用反应性human, mouse, rat
免疫原

CatNo: Ag6552

Product name: Recombinant human FEN1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-380 aa of BC000323

Sequence: MGIQGLAKLIADVAPSAIRENDIKSYFGRKVAIDASMSIYQFLIAVRQGGDVLQNEEGETTSHLMGMFYRTIRMMENGIKPVYVFDGKPPQLKSGELAKRSERRAEAEKQLQQAQAAGAEQEVEKFTKRLVKVTKQHNDECKHLLSLMGIPYLDAPSEAEASCAALVKAGKVYAAATEDMDCLTFGSPVLMRHLTASEAKKLPIQEFHLSRILQELGLNQEQFVDLCILLGSDYCESIRGIGPKRAVDLIQKHKSIEEIVRRLDPNKYPVPENWLHKEAHQLFLEPEVLDPESVELKWSEPNEEELIKFMCGEKQFSEERIRSGVKRLSKSRQGSTQGRLDDFFKVTGSLSSAKRKEPEPKGSTKKKAKTGAAGKFKRGK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 flap structure-specific endonuclease 1
别名 hFEN-1, hFEN 1, Flap structure-specific endonuclease 1, FEN-1, FEN 1
计算分子量 43 kDa
观测分子量 48 kDa
GenBank蛋白编号BC000323
基因名称 FEN1
Gene ID (NCBI) 2237
RRIDAB_2102780
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP39748
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FEN1(Flap endonuclease-1) is the prototypical member of the 5'-nuclease superfamily, whose activities span a range of cellular pathways involved in DNA replication and genome maintenance (PMID: 22118811, 21496641, 20929870). FEN1 is a structure-selective metallonuclease essential for Okazaki fragment maturation through efficient removal of 5' flaps resulting from strand displacement during lagging-strand synthesis (PMID: 8144677, 9081985). FEN1 is overexpressed in multiple cancer types, and has been suggested both as a biomarker relating to prognosis and disease progression and as a potential therapeutic target (PMID: 19010819, 16879693, 19596913, 27526030).

实验方案

Product Specific Protocols
WB protocol for FEN1 antibody 14768-1-APDownload protocol
IHC protocol for FEN1 antibody 14768-1-APDownload protocol
IF protocol for FEN1 antibody 14768-1-APDownload protocol
IP protocol for FEN1 antibody 14768-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB,IF

Adv Sci (Weinh)

Primate-Specific DAZ Regulates Translation of Cell Proliferation-Related mRNAs and is Essential for Maintenance of Spermatogonia

Authors - Ningjing Ou
View Article
ratWB

Nat Commun

Cis- and trans-resveratrol have opposite effects on histone serine-ADP-ribosylation and tyrosine induced neurodegeneration.

Authors - Megha Jhanji
View Article
mouseWB

Cell Death Dis

DNA polymerase beta connects tumorigenicity with the circadian clock in liver cancer through the epigenetic demethylation of Per1

Authors - Siyu Chen
View Article
mouseWB

J Med Chem

CJ2: A Novel Potent Platinum(IV) Prodrug Enhances Chemo-Immunotherapy by Facilitating PD-L1 Degradation in the Cytoplasm and Cytomembrane

Authors - Renming Fan
View Article
humanWB

J Mol Cell Biol

SUMO-1 modification of FEN1 facilitates its interaction with Rad9-Rad1-Hus1 to counteract DNA replication stress.

Authors - Xiaoli Xu
View Article
humanWB

Biology (Basel)

In Situ Peroxidase Labeling Followed by Mass-Spectrometry Reveals TIA1 Interactome.

Authors - Olga Gourdomichali
View Article