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验证数据展示

  • WB analysis of MCF-7 using 66258-1-Ig (same clone as 66258-1-PBS)

    WB analysis of MCF-7 using 66258-1-Ig (same clone as 66258-1-PBS)

    WB result of FAK antibody (66258-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FAK transfected MCF-7 cells. This data was developed using the same antibody clone with 66258-1-PBS in a different storage buffer formulation.

  • WB analysis using 66258-1-Ig (same clone as 66258-1-PBS)

    WB analysis using 66258-1-Ig (same clone as 66258-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66258-1-Ig (FAK antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66258-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66258-1-Ig (same clone as 66258-1-PBS)

    IHC staining of human breast cancer using 66258-1-Ig (same clone as 66258-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66258-1-Ig (FAK antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66258-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FAK antibody included
  • IHC staining of human breast cancer using 66258-1-Ig (same clone as 66258-1-PBS)

    IHC staining of human breast cancer using 66258-1-Ig (same clone as 66258-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66258-1-Ig (FAK antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66258-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FAK antibody included
  • IF Staining of HUVEC using 66258-1-Ig (same clone as 66258-1-PBS)

    IF Staining of HUVEC using 66258-1-Ig (same clone as 66258-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using FAK antibody (66258-1-Ig, Clone: 2C5B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66258-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

FAK Monoclonal antibody, PBS Only

FAK Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66258-1-PBS

产品说明书

CloneNo. 2C5B9

宿主/亚型

Mouse / IgG2c

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

PTK2, 2C5B9, EC:2.7.10.2, FADK 1, FAK1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy FAK Ready-To-Use IHC Kit. FAK primary antibody included.

产品信息

66258-1-PBS targets FAK in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag17966

Product name: Recombinant human FAK protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 381-678 aa of BC028733

Sequence: ITAMAGSIYPGQASLLDQTDSWNHRPQEIAMWQPNVEDSTVLDLRGIGQVLPTHLMEERLIRQQQEMEEDQRWLEKEERFLKPDVRLSRGSIDREDGSLQGPIGNQHIYQPVGKPDPAAPPKKPPRPGAPGHLGSLASLSSPADSYNEGVKLKPQEISPPPTANLDRSNDKVYENVTGLVKAVIEMSSKIQPAPPEEYVPMVKEVGLALRTLLATVDETIPLLPASTHREIEMAQKLLNSDLGELINKMKLAQQYVMTSLQQEYKKQMLTAAHALAVDAKNLLDVIDQARLKMLGQTR

种属同源性预测
宿主/亚型 Mouse / IgG2c
抗体类别 Monoclonal
产品类型 Antibody
全称 PTK2 protein tyrosine kinase 2
别名 PTK2, 2C5B9, EC:2.7.10.2, FADK 1, FAK1
计算分子量 1052 aa, 119 kDa
观测分子量 110 kDa
GenBank蛋白编号BC028733
基因名称 FAK
Gene ID (NCBI) 5747
RRIDAB_2881646
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ05397
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FAK (Focal adhesion kinase 1) is also named as FAK1, FADK, pp125FAK, FAK and belongs to the protein kinase superfamily. It is a critical tyrosine kinase that modulates cell adhesion, migration, proliferation and survival in response to extracellular signals (PMID:19664602). It also acts as a pivotal signal 'integrator', controlling and coordinating cellular responses that include cell migration, survival, proliferation and, epithelial tissue repair after DNA damage (PMID:20966971). This protein has some isoforms produced by alternative promoter usage and alternative splicing, and the range of the molecular weights are 100-120kD and 40-60kD.