验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, mouse liver tissue, HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12222-1-AP targets DPEP1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, monkey |
| 免疫原 |
CatNo: Ag2907 Product name: Recombinant human DPEP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-385 aa of BC017023 Sequence: MWSGWWLWPLVAVCTADFFRDEAERIMRDSPVIDGHNDLPWQLLDMFNNRLQDERANLTTLAGTHTNIPKLRAGFVGGQFWSVYTPCDTQNKDAVRRTLEQMDVVHRMCRMYPETFLYVTSSAGIRQAFREGKVASLIGVEGGHSIDSSLGVLRALYQLGMRYLTLTHSCNTPWADNWLVDTGDSEPQSQGLSPFGQRVVKELNRLGVLIDLAHVSVATMKATLQLSRAPVIFSHSSAYSVCASRRNVPDDVLRLVKQTDSLVMVNFYNNYISCTNKANLSQVADHLDHIKEVAGARAVGFGGDFDGVPRVPEGLEDVSKYPDLIAELLRRNWTEAEVKGALADNLLRVFEAVEQASNLTQAPEEEPIPLDQLGGSCRTHYGYSS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | dipeptidase 1 (renal) |
| 别名 | MBD1, hRDP, EC:3.5.2.6, EC:3.4.13.19, Dehydropeptidase-I |
| 计算分子量 | 411 aa, 46 kDa |
| 观测分子量 | 46 kDa |
| GenBank蛋白编号 | BC017023 |
| 基因名称 | DPEP1 |
| Gene ID (NCBI) | 1800 |
| RRID | AB_2877837 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P16444 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DPEP1(Dipeptidase 1) is also named as MDP, RDP and belongs to the peptidase M19 family. It is a glycoprotein that plays an important role in the regulation of leukotriene activity. DPEP1 converts lekotriene D4 to leukotriene E4.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DPEP1 antibody 12222-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Dipeptidase-1 Is an Adhesion Receptor for Neutrophil Recruitment in Lungs and Liver.
| ||
Sci Adv Dexamethasone sensitizes to ferroptosis by glucocorticoid receptor-induced dipeptidase-1 expression and glutathione depletion.
| ||
J Clin Invest Renal immune surveillance and dipeptidase-1 contribute to contrast-induced acute kidney injury. | ||
Nat Commun A single genetic locus controls both expression of DPEP1/CHMP1A and kidney disease development via ferroptosis.
| ||
Int Immunopharmacol Dipeptidase 1 promotes ferroptosis in renal tubular epithelial cells in diabetic nephropathy via inhibition of the GSH/GPX4 axis |
