验证数据展示

WB analysis using 12216-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12216-1-AP (Cathepsin B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC staining of human ovary tumor using 12216-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12216-1-AP (Cathepsin B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with Cathepsin B antibody included
FC experiment of MCF-7 using 12216-1-AP
1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human Cathepsin B (12216-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
WB analysis of HepG2 using 12216-1-AP
WB result of Cathepsin B antibody (12216-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cathepsin B transfected HepG2 cells.
IP experiment of HepG2 using 12216-1-AP
IP result of anti-Cathepsin B (IP:12216-1-AP, 4ug; Detection:12216-1-AP 1:3000) with HepG2 cells lysate 2000 ug.
IF Staining of A375 using 12216-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed A375 cells using Cathepsin B antibody (12216-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO验证

Cathepsin B Polyclonal antibody

Cathepsin B Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 12216-1-AP

产品说明书

引用文献(89)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (6)

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (1)

别名

CTSB, APP secretase, APPS, Cat B, Cathepsin B heavy chain

缓冲液配方: PBS, Azide, Glycerol

PBS, Azide, Glycerol

偶联物: Unconjugated

Unconjugated

规格	价格	库存

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概述

经过测试的应用
产品信息
发表文章 (89)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	A375 cells, HepG2 cells, HT-1080 cells
Positive IP detected in	HepG2 cells
Positive IHC detected in	human ovary tumor tissue, human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A375 cells
Positive FC (Intra) detected in	MCF-7 cells

Planning an IHC experiment? We recommend our IHCeasy Cathepsin B Ready-To-Use IHC Kit. Cathepsin B primary antibody included.

应用	推荐稀释比
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 1 publications below
WB	See 69 publications below
IHC	See 9 publications below
IF	See 21 publications below

产品信息

12216-1-AP targets Cathepsin B in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

经测试应用	WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
文献引用应用	WB, IHC, IF
经测试反应性	human
文献引用反应性	human, mouse, rat, pig, zebrafish, bovine, frog
免疫原	CatNo: Ag2898 Product name: Recombinant human Cathepsin B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-339 aa of BC010240 Sequence: MWQLWASLCCLLVLANARSRPSFHPVSDELVNYVNKRNTTWQAGHNFYNVDMGYLKRLCGTFLGGPKPPQRVMFTEDLKLPASFDAREQWPQCPTIKEIRDQGSCGSCWAFGAVEAISDRICIHTNAHVSVEVSAEDLLTCCGSMCGDGCNGGYPAEAWNFWTRKGLVSGGLYESHVGCRPYSIPPCEHHVNGSRPPCTGEGDTPKCSKICEPGYSPTYKQDKHYGYNSYSVSNSEKDIMAEIYKNGPVEGAFSVYSDFLLYKSGVYQHVTGEMMGGHAIRILGWGVENGTPYWLVANSWNTDWGDNGFFKILRGQDHCGIESEVVAGIPRTDQYWEKI 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	cathepsin B
别名	CTSB, APP secretase, APPS, Cat B, Cathepsin B heavy chain
计算分子量	339 aa, 38 kDa
观测分子量	25 kDa, 31 kDa, 43 kDa
GenBank蛋白编号	BC010240
基因名称	Cathepsin B
Gene ID (NCBI)	1508
RRID	AB_2086929
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	P07858
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

CTSB(Cathepsin B) is also named as CPSB and belongs to the peptidase C1 family. It participates in intracellular degradation and turnover of proteins. Cathepsin B precursors found in human malignant ascites fluid do not possess mannose-rich carbohydrates suggesting that a defect in the post translational processing of carbohydrate moieties on tumor. Cathepsin B exists as both glycosylated and unglycosylated forms(PMID:1637335). In rat macrophages and hepatocytes pro- cathepsin B is 39 kDa (unglycosylated =35 kDa), whereas in human fibroblasts procathepsin B is 44.5-46kDa (unglycosylated=39kDa)(PMID:2097084). It can be detected the 43 kDa form of pro-CTSB, 31 kDa and 25 kDa mature forms in mouse brain by western blot(PMID:20616152).

实验方案

Product Specific Protocols
IF protocol for Cathepsin B antibody 12216-1-AP	Download protocol
IHC protocol for Cathepsin B antibody 12216-1-AP	Download protocol
IP protocol for Cathepsin B antibody 12216-1-AP	Download protocol
WB protocol for Cathepsin B antibody 12216-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
mouse	WB,IF	Nature Neurotoxic microglia promote TDP-43 proteinopathy in progranulin deficiency. Authors - Jiasheng Zhang View Article
rat	WB	Cell Death Differ NLRX1 mediated impaired microglial phagocytosis of NETs in cerebral ischemia and reperfusion injury Authors - Jialing Peng View Article
human	WB	Autophagy Atractylenolide I inhibits angiogenesis and reverses sunitinib resistance in clear cell renal cell carcinoma through ATP6V0D2-mediated autophagic degradation of EPAS1/HIF2α Authors - Qinyu Li View Article
human	IF	Cell Death Differ Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration Authors - Huaizhen Liang View Article
mouse	WB,IHC	Aging Cell Growth differentiation factor 11 accelerates liver senescence through the inhibition of autophagy Authors - Jian Sun View Article
human	WB	Redox Biol Inhibition of STAT3-ferroptosis negative regulatory axis suppresses tumor growth and alleviates chemoresistance in gastric cancer. Authors - Shumin Ouyang View Article

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验证数据展示

WB analysis using 12216-1-AP

IHC staining of human ovary tumor using 12216-1-AP

FC experiment of MCF-7 using 12216-1-AP

WB analysis of HepG2 using 12216-1-AP

IP experiment of HepG2 using 12216-1-AP

IF Staining of A375 using 12216-1-AP

Cathepsin B Polyclonal antibody

Cathepsin B Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Neurotoxic microglia promote TDP-43 proteinopathy in progranulin deficiency.

NLRX1 mediated impaired microglial phagocytosis of NETs in cerebral ischemia and reperfusion injury

Atractylenolide I inhibits angiogenesis and reverses sunitinib resistance in clear cell renal cell carcinoma through ATP6V0D2-mediated autophagic degradation of EPAS1/HIF2α

Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration

Growth differentiation factor 11 accelerates liver senescence through the inhibition of autophagy

Inhibition of STAT3-ferroptosis negative regulatory axis suppresses tumor growth and alleviates chemoresistance in gastric cancer.