验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, L02 cells, mouse liver tissue, rat liver tissue |
| Positive IHC detected in | human liver cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11418-2-AP targets COX5B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, monkey, goat |
| 免疫原 |
CatNo: Ag1986 Product name: Recombinant human COX5B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-129 aa of BC006229 Sequence: MASRLLRGAGTLAAQALRARGPSGAAAMRSMASGGGVPTDEEQATGLEREIMLAAKKGLDPYNVLAPKGASGTREDPNLVPSISNKRIVGCICEEDNTSVVWFWLHKGEAQRCPRCGAHYKLVPQQLAH 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cytochrome c oxidase subunit Vb |
| 别名 | COXVB |
| 计算分子量 | 129 aa, 14 kDa |
| 观测分子量 | 14 kDa |
| GenBank蛋白编号 | BC006229 |
| 基因名称 | COX5B |
| Gene ID (NCBI) | 1329 |
| RRID | AB_2245376 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P10606 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
COX5B belongs to the cytochrome c oxidase subunit 5B family. It is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. COX5B and other 3 genes CKB, HBB, MBP are involved in respiration.(PMID:21295140)
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for COX5B antibody 11418-2-AP | Download protocol |
| IHC protocol for COX5B antibody 11418-2-AP | Download protocol |
| WB protocol for COX5B antibody 11418-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Mitochondrial Protein Synthesis Adapts to Influx of Nuclear-Encoded Protein.
| ||
Nat Immunol Mitochondrial respiratory-chain adaptations in macrophages contribute to antibacterial host defense. | ||
Cell Metab Enhanced Respiratory Chain Supercomplex Formation in Response to Exercise in Human Skeletal Muscle. | ||
Mol Cell Global mitochondrial protein import proteomics reveal distinct regulation by translation and translocation machinery |
