验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, mouse lung tissue, HeLa cells, A431 cells, mouse kidney tissue |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | mouse brain tissue, human lung tissue, mouse testis tissue, human skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15761-1-AP targets CHMP1A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag8389 Product name: Recombinant human CHMP1A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-196 aa of BC007527 Sequence: MDDTLFQLKFTAKQLEKLAKKAEKDSKAEQAKVKKALLQKNVECARVYAENAIRKKNEGVNWLRMASRVDAVASKVQTAVTMKGVTKNMAQVTKALDKALSTMDLQKVSSVMDRFEQQVQNLDVHTSVMEDSMSSATTLTTPQEQVDSLIMQIAEENGLEVLDQLSQLPEGASAVGESSVRSQEDQLSRRLAALRN 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromatin modifying protein 1A |
| 别名 | KIAA0047, hVps46-1, hVps46 1, Chromatin-modifying protein 1a, chromatin modifying protein 1A |
| 计算分子量 | 196 aa, 22 kDa |
| 观测分子量 | 33 kDa, 25 kDa |
| GenBank蛋白编号 | BC007527 |
| 基因名称 | CHMP1A |
| Gene ID (NCBI) | 5119 |
| RRID | AB_2229399 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9HD42 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Charged multivesicular body protein 1A (CHMP1A), also known as chromatin modifying protein 1A, is a member of the ESCRT-III (endosomal sorting complex required for transport III) complex which is involved in degradation of internalized transmembrane receptor proteins and formation of multivesicular bodies (MVBs) (PMID: 16730941). CHMP1A has both nuclear and cytoplasmic distributions (PMID: 11559748; 11559747). Subcellular localization of CHMP1A seems to vary depending on the cell type (PMID: 23023333). CHMP1A is required for MVBs formation and has a role in stable gene silencing within the nucleus. Besides, the tumor suppressor role of CHMP1A has also been reported (PMID: 18787405; 22261332).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CHMP1A antibody 15761-1-AP | Download protocol |
| IHC protocol for CHMP1A antibody 15761-1-AP | Download protocol |
| IF protocol for CHMP1A antibody 15761-1-AP | Download protocol |
| IP protocol for CHMP1A antibody 15761-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation. | ||
J Extracell Vesicles Accessory ESCRT-III proteins are conserved and selective regulators of Rab11a-exosome formation | ||
Nat Commun A single genetic locus controls both expression of DPEP1/CHMP1A and kidney disease development via ferroptosis.
| ||
Nat Commun Mechanism of Ca²⁺-triggered ESCRT assembly and regulation of cell membrane repair. | ||
Cell Rep The ESCRT-III Protein CHMP1A Mediates Secretion of Sonic Hedgehog on a Distinctive Subtype of Extracellular Vesicles.
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