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验证数据展示

  • WB analysis of Jurkat using 10394-1-AP (same clone as 10394-1-PBS)

    WB analysis of Jurkat using 10394-1-AP (same clone as 10394-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 10394-1-AP (CFLAR/FLIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10394-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 10394-1-AP (same clone as 10394-1-PBS)

    WB analysis of HepG2 using 10394-1-AP (same clone as 10394-1-PBS)

    WB result of CFLAR/FLIP antibody (10394-1-AP; 1:500; incubated at room temperature for 1.5 hours) with sh-Control and sh-CFLAR/FLIP transfected HepG2 cells. This data was developed using the same antibody clone with 10394-1-PBS in a different storage buffer formulation.

  • IP experiment of Jurkat using 10394-1-AP (same clone as 10394-1-PBS)

    IP experiment of Jurkat using 10394-1-AP (same clone as 10394-1-PBS)

    IP result of anti-CFLAR/FLIP (IP:10394-1-AP, 4ug; Detection:10394-1-AP 1:500) with Jurkat cells lysate 4000ug. This data was developed using the same antibody clone with 10394-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 10394-1-AP (same clone as 10394-1-PBS)

    IF Staining of HeLa using 10394-1-AP (same clone as 10394-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CFLAR/FLIP antibody (10394-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10394-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse skeletal muscle using 10394-1-AP (same clone as 10394-1-PBS)

    IHC staining of mouse skeletal muscle using 10394-1-AP (same clone as 10394-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 10394-1-AP (CFLAR/FLIP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10394-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CFLAR/FLIP antibody included
  • IHC staining of mouse skeletal muscle using 10394-1-AP (same clone as 10394-1-PBS)

    IHC staining of mouse skeletal muscle using 10394-1-AP (same clone as 10394-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 10394-1-AP (CFLAR/FLIP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10394-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CFLAR/FLIP antibody included
  • Featured Product
  • KD/KO验证

CFLAR/FLIP Polyclonal antibody, PBS Only

CFLAR/FLIP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 10394-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, ELISA

别名

CFLAR, c FLIP, c FLIPL, c FLIPR, c FLIPS

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CFLAR/FLIP Ready-To-Use IHC Kit. CFLAR/FLIP primary antibody included.

产品信息

10394-1-PBS targets CFLAR/FLIP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag0593

Product name: Recombinant human CFLAR protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 31-184 aa of BC001602

Sequence: DVVPPNVRDLLDILRERGKLSVGDLAELLYRVRRFDLLKRILKMDRKAVETHLLRNPHLVSDYRVLMAEIGEDLDKSDVSSLIFLMKDYMGRGKISKEKSFLDLVVELEKLNLVAPDQLDLLEKCLKNIHRIDLKTKIQKYKQSVQGAGTSYRN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CASP8 and FADD-like apoptosis regulator
别名 CFLAR, c FLIP, c FLIPL, c FLIPR, c FLIPS
计算分子量 52 kDa
观测分子量 20-35 kDa, 50-55 kDa
GenBank蛋白编号BC001602
基因名称 FLIP
Gene ID (NCBI) 8837
RRIDAB_2081087
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO15519
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CFLAR, also named as CASH, CASP8AP1, CLARP, MRIT, Casper, c-FLIP and I-FLICE, belongs to the peptidase C14A family. It is an apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. CFLAR acts as an inhibitor of TNFRSF6 mediated apoptosis. It can be cleaved to be 2 fragments P43 and P12. P43 is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. CFLAR lacks enzymatic (caspase) activity.

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