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验证数据展示

  • WB analysis of HeLa using 60301-1-Ig

    WB analysis of HeLa using 60301-1-Ig

    WB result of BID antibody (60301-1-Ig, 1:8000) with si-Control and si-BID transfected HeLa cells.

  • WB analysis of HeLa using 60301-1-Ig

    WB analysis of HeLa using 60301-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 60301-1-Ig (BID Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 60301-1-Ig

    WB analysis of Jurkat using 60301-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 60301-1-Ig (BID Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 60301-1-Ig

    IHC staining of human colon cancer using 60301-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60301-1-Ig (BID Antibody) at dilution of 1:900 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BID antibody included
  • IHC staining of human colon cancer using 60301-1-Ig

    IHC staining of human colon cancer using 60301-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60301-1-Ig (BID Antibody) at dilution of 1:900 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BID antibody included
  • IF Staining of HeLa using 60301-1-Ig

    IF Staining of HeLa using 60301-1-Ig

    Immunofluorescent analysis of HeLa cells using 60301-1-Ig (BID antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

  • Featured Product
  • KD/KO验证

BID Monoclonal antibody

BID Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 60301-1-Ig

产品说明书

CloneNo. 2A2E5

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

p22 BID, 2A2E5, BH3 interacting domain death agonist, BH3-interacting domain death agonist, BH3-interacting domain death agonist p11

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite®594 HRP
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy BID Ready-To-Use IHC Kit. BID primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:200-1:1800
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60301-1-Ig targets BID in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag21005

Product name: Recombinant human BID protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1-195 aa of BC009197

Sequence: MDCEVNNGSSLRDECITNLLVFGFLQSCSDNSFRRELDALGHELPVLAPQWEGYDELQTDGNRSSHSRLGRIEADSESQEDIIRNIARHLAQVGDSMDRSIPPGLVNGLALQLRNTSRSEEDRNRDLATALEQLLQAYPRDMEKEKTMLVLALLLAKKVASHTPSLLRDVFHTTVNFINQNLRTYVRSLARNGMD

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 BH3 interacting domain death agonist
别名 p22 BID, 2A2E5, BH3 interacting domain death agonist, BH3-interacting domain death agonist, BH3-interacting domain death agonist p11
计算分子量 22 kDa
观测分子量 22 kDa
GenBank蛋白编号BC009197
基因名称 BID
Gene ID (NCBI) 637
RRIDAB_2881416
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP55957
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BID, also named as p22 BID, can be cleaved into p11 BID, p13 BID and p15 BID. It is pro-apoptotic molecules. The major proteolytic product p15 BID allows the release of cytochrome c. BID-L, BID-EL and BID-ES induce ICE-like proteases and apoptosis. BID-S does not induce apoptosis. BID counters the protective effect of Bcl-2. (PMID:14583606).

实验方案

Product Specific Protocols
WB protocol for BID antibody 60301-1-IgDownload protocol
IHC protocol for BID antibody 60301-1-IgDownload protocol
IF protocol for BID antibody 60301-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols