验证数据展示
经过测试的应用
| Positive WB detected in | mouse heart tissue, mouse skeletal muscle tissue, mouse thymus tissue |
| Positive IP detected in | mouse heart tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
| IHC | See 1 publications below |
产品信息
11032-1-AP targets Alpha cardiac muscle actin in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag1471 Product name: Recombinant human ACTC1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-377 aa of BC009978 Sequence: MCDDEETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDEAGPSIVHRKCF 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | actin, alpha, cardiac muscle 1 |
| 别名 | ACTC1, Alpha actin, ACTC, ACTC 1, ACTC1 cardiac muscle 1 |
| 计算分子量 | 377 aa, 42 kDa |
| 观测分子量 | 42 kDa |
| GenBank蛋白编号 | BC009978 |
| 基因名称 | ACTC1 |
| Gene ID (NCBI) | 70 |
| RRID | AB_2242376 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P68032 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Alpha cardiac muscle actin antibody 11032-1-AP | Download protocol |
| IP protocol for Alpha cardiac muscle actin antibody 11032-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int J Biol Sci MnSOD Lysine 68 acetylation leads to cisplatin and doxorubicin resistance due to aberrant mitochondrial metabolism. | ||
Stem Cells Dev A model of early human embryonic stem cell differentiation reveals inter- and intracellular changes on transition to squamous epithelium. | ||
Onco Targets Ther Clinical Significance and Oncogenic Activity of GRWD1 Overexpression in the Development of Colon Carcinoma. |
