验证数据展示
经过测试的应用
| Positive WB detected in | Ag30676 |
| Positive ELISA detected in | Recombinant protein |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Enzyme-linked Immunosorbent Assay (ELISA) | ELISA : |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67666-1-Ig targets SARS-CoV-2 Nucleocapsid Phosphoprotein in WB, ELISA applications and shows reactivity with virus samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | virus |
| 文献引用反应性 | mouse |
| 免疫原 |
CatNo: Ag30676 Product name: Recombinant virus 2019-nCOV nucleocapsid phosphoprotein protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-419 aa of NC_045512 Sequence: MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | COVID-19 N Protein |
| 别名 | nucleocapsid phosphoprotein, 1B3C3, sars cov 2 |
| GenBank蛋白编号 | NC_045512 |
| 基因名称 | COVID-19 N Protein |
| Gene ID (NCBI) | 43740575 |
| RRID | AB_2882862 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The nucleocapsid (N) protein has multiple functions including formation of nucleocapsids, signal transduction virus budding, RNA replication, and mRNA transcription. N protein is an important antigen for coronavirus, and it is normally highly conserved, with a molecular weight of about 50 kDa. it can be used as a marker in diagnostic assays due to its high immunogenicity (PMID: 32416961, PMID: 32235387).67666-1-Ig can be used as capture antibody. 67666-2-Ig can be used as detection antibody.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SARS-CoV-2 Nucleocapsid Phosphoprotein antibody 67666-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Biosens Bioelectron Electronic field effect detection of SARS-CoV-2 N-protein before the onset of symptoms. | ||
J Virol Palmitoylation of SARS-CoV-2 Envelope protein is central to virus particle formation |