验证数据展示
经过测试的应用
| Positive ELISA detected in | Recombinant protein |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Enzyme-linked Immunosorbent Assay (ELISA) | ELISA : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IF | See 1 publications below |
产品信息
28882-1-AP targets SARS-CoV-2 Membrane Glycoprotein in WB, IF, ELISA applications and shows reactivity with virus samples.
| 经测试应用 | ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | virus |
| 免疫原 |
CatNo: Ag30691 Product name: Recombinant virus membrane glycoprotein protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 101-222 aa of NC_045512 Sequence: RLFARTRSMWSFNPETNILLNVPLHGTILTRPLLESELVIGAVILRGHLRIAGHHLGRCDIKDLPKEITVATSRTLSYYKLGASQRVAGDSGFAAYSRYRIGNYKLNTDHSSSSDNIALLVQ 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | COVID-19 M Protein |
| 别名 | 2019-nCOV membrane glycoprotein, M protein, membrane glycoprotein |
| GenBank蛋白编号 | NC_045512 |
| 基因名称 | SARS-CoV-2 Membrane Protein |
| Gene ID (NCBI) | 43740571 |
| RRID | AB_2881226 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P0DTC5 |
| 储存缓冲液 | PBS with 0.02% sodium azide, 50% glycerol, and 0.05% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
In the coronaviruses, the membrane (M) protein was reported to be the most abundant viral protein expressed during infection and a key protein in the assembly of both naked and enveloped virus particles (PMID: 11967315). The M glycoprotein is conserved across the β-coronaviruses. The multiple sequence alignment shows a remarkable similarity (98% identity) among the Sars-CoV-2 M variants and the sequences from Bat and Pangolin isolates (PMID:32596311). The M protein is predicted to contain a triple-spanning transmembrane (TM) region, a single N-glycosylation site near its N-terminus that is in the exterior of the virion, and a long C-terminal region in the interior (PMID: 15626342).
发表文章
| Species | Application | Title |
|---|---|---|
J Virol Palmitoylation of SARS-CoV-2 Envelope protein is central to virus particle formation |