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验证数据展示

  • IF Staining of HEK-293T using 29286-1-AP

    IF Staining of HEK-293T using 29286-1-AP

    Immunofluorescent analysis of 4% PFA fixed HEK293T cells overexpressing ACE2 and TMPRSS using NSP5 antibody (29286-1-AP, green) at dilution of 1:200. Cells were infected with virus (dsRNA, in red, MOI 0.01, 48hpi). The images were credited by Dr. Irene Chen, Gladstone Institute.

  • ELISA experiment using 29286-1-AP

    ELISA experiment using 29286-1-AP

    SARS-CoV-2 Envelope protein Antibody (29286-1-AP) tested by ELISA. SARS-COV-2 NSP5 protein was coated onto microtiter plates at 1 µg/well and then incubated with a dilution series of SARS-COV-2 NSP5 protein Antibody (29286-1-AP). Bound antibodies were detected with HRP conjugated anti-Rabbit IgG followed by incubation with HRP Substrate and then measuring the resulting absorbance at 450 nm.

SARS-COV-2 NSP5 Polyclonal antibody

SARS-COV-2 NSP5 Polyclonal Antibody for IF, ELISA
Cat No. 29286-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

virus and More (1)

应用

IF, ELISA and More (1)

别名

SARS-COV-2 NSP5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHEK-293T cells
Positive ELISA detected inN/A

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
Enzyme-linked Immunosorbent Assay (ELISA)ELISA :
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29286-1-AP targets SARS-COV-2 NSP5 in WB, IF/ICC, ELISA applications and shows reactivity with virus samples.

经测试应用 IF, ELISA Application Description
文献引用应用WB, IF
经测试反应性 virus
文献引用反应性human
免疫原

CatNo: Ag30934

Product name: Recombinant sars-cov-2 NSP5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-306 aa of YP_009742612

Sequence: SGFRKMAFPSGKVEGCMVQVTCGTTTLNGLWLDDVVYCPRHVICTSEDMLNPNYEDLLIRKSNHNFLVQAGNVQLRVIGHSMQNCVLKLKVDTANPKTPKYKFVRIQPGQTFSVLACYNGSPSGVYQCAMRPNFTIKGSFLNGSCGSVGFNIDYDCVSFCYMHHMELPTGVHAGTDLEGNFYGPFVDRQTAQAAGTDTTITVNVLAWLYAAVINGDRWFLNRFTTTLNDFNLVAMKYNYEPLTQDHVDILGPLSAQTGIAVLDMCASLKELLQNGMNGRTILGSALLEDEFTPFDVVRQCSGVTFQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ORF1ab
别名 SARS-COV-2 NSP5
GenBank蛋白编号YP_009742612
基因名称 SARS-COV-2 NSP8
Gene ID (NCBI) 43740578
RRIDAB_2918269
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

发表文章

SpeciesApplicationTitle
humanWB

J Cell Biol

SARS-CoV-2 NSP3/4 control formation of replication organelle and recruitment of RNA polymerase NSP12

Authors - Jinping Yang
View Article
IF

Cell Rep

Pressure to evade cell-autonomous innate sensing reveals interplay between mitophagy, IFN signaling, and SARS-CoV-2 evolution

Authors - Jae Seung Lee
View Article
humanWB

J Biol Chem

E3 ubiquitin ligase ZBTB25 suppresses beta coronavirus infection through ubiquitination of the main viral protease MPro

Authors - Travis B Lear
View Article