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验证数据展示

  • WB analysis of HeLa using 18144-1-AP

    WB analysis of HeLa using 18144-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 18144-1-AP (RPA4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 18144-1-AP

    IP experiment of HeLa using 18144-1-AP

    IP result of anti-RPA4 (IP:18144-1-AP, 4ug; Detection:18144-1-AP 1:300) with HeLa cells lysate 3400ug.

RPA4 Polyclonal antibody

RPA4 Polyclonal Antibody for WB, IP, ELISA
Cat No. 18144-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, ELISA

别名

HSU24186, RF-A protein 4, RP A p30, RPA 4, RP-A p30

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, mouse spleen tissue
Positive IP detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18144-1-AP targets RPA4 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag12800

Product name: Recombinant human RPA4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-261 aa of BC069791

Sequence: MSKSGFGSYGSISAADGASGGSDQLCERDATPAIKTQRPKVRIQDVVPCNVNQLLSSTVFDPVFKVRGIIVSQVSIVGVIRGAEKASNHICYKIDDMTAKPIEARQWFGREKVKQVTPLSVGVYVKVFGILKCPTGTKSLEVLKIHVLEDMNEFTVHILETVNAHMMLDKARRDTTVESVPVSPSEVNDAGDNDESHRNFIQDEVLRLIHECPHQEGKSIHELRAQLCDLSVKAIKEAIDYLTVEGHIYPTVDREHFKSAD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 replication protein A4, 34kDa
别名 HSU24186, RF-A protein 4, RP A p30, RPA 4, RP-A p30
计算分子量 261 aa, 29 kDa
观测分子量 29-32 kDa
GenBank蛋白编号BC069791
基因名称 RPA4
Gene ID (NCBI) 29935
RRIDAB_2180970
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13156
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RPA4, also named as RP-A p30, is a 261 amino acid protein, which belongs to the replication factor A protein 2 family. RPA4 is preferentially expressed in placental and colon mucosa. RPA4 as part of the alternative replication protein A complex, aRPA, binds single-stranded DNA and probably plays a role in DNA repair. This protein, Rpa4, has 47% identity with Rpa2, the 34-kDa subunit of RPA. (PMID: 7760808)

实验方案

Product Specific Protocols
IP protocol for RPA4 antibody 18144-1-APDownload protocol
WB protocol for RPA4 antibody 18144-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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