验证数据展示

WB analysis of MCF-7 using 16215-1-AP
WB result of ACAT1 antibody (16215-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACAT1 transfected MCF-7 cells.
WB analysis using 16215-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16215-1-AP (ACAT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC staining of human liver using 16215-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16215-1-AP (ACAT1 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with ACAT1 antibody included
FC experiment of HeLa using 16215-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ACAT1 (16215-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
IP experiment of mouse heart using 16215-1-AP
IP result of anti-ACAT1 (IP:16215-1-AP, 4ug; Detection:16215-1-AP 1:500) with mouse heart tissue lysate 4400ug.
IHC staining of human liver using 16215-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 16215-1-AP (ACAT1 antibody) at dilution of 1:100 (under 10x lens).
Ready-to-use IHC kits available with ACAT1 antibody included
IHC staining of human colon using 16215-1-AP
Immunohistochemical analysis of paraffin-embedded human colon slide using 16215-1-AP (ACAT1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with ACAT1 antibody included
IF Staining of HeLa using 16215-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ACAT1 antibody (16215-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

Featured Product
KD/KO验证

ACAT1 Polyclonal antibody

ACAT1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 16215-1-AP

产品说明书

引用文献(38)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (2)

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (2)

别名

ACAT, Acetoacetyl CoA thiolase, Acetoacetyl-CoA thiolase, Acetyl-CoA acetyltransferase, mitochondrial, EC:2.3.1.9

缓冲液配方: PBS and Azide

PBS and Azide

偶联物: Unconjugated

Unconjugated

CoraLite® Plus 488

规格	价格	库存

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概述

经过测试的应用
产品信息
发表文章 (38)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	mouse heart tissue, Caco-2 cells, human skeletal muscle tissue, MCF-7 cells, rat skeletal muscle tissue, mouse colon tissue
Positive IP detected in	mouse heart tissue
Positive IHC detected in	human colon, human liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells
Positive FC (Intra) detected in	HeLa cells

Planning an IHC experiment? We recommend our IHCeasy ACAT1 Ready-To-Use IHC Kit. ACAT1 primary antibody included.

Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

应用	推荐稀释比
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 2 publications below
WB	See 33 publications below
IHC	See 6 publications below
IF	See 5 publications below
IP	See 2 publications below
CoIP	See 1 publications below

产品信息

16215-1-AP targets ACAT1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用	WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
文献引用应用	WB, IHC, IF, IP, CoIP
经测试反应性	human, mouse
文献引用反应性	human, mouse, rat, rabbit
免疫原	CatNo: Ag9215 Product name: Recombinant human ACAT1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-162 aa of BC010942 Sequence: MAVLPALLRSGARSRSPLLRRLVQEIRYVERSYVSKPTLKEVVIVSATRTPIGSFLGSLSLLPATKLGSIAIQGAIEKAGIPKEEVKEAYMGNVLQGGEGQAPTRQAVLGAGLPISTPCTTINKVCASGMKAIMMASQSLMCGHQIKQETGSLAKICCHVRR 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	acetyl-Coenzyme A acetyltransferase 1
别名	ACAT, Acetoacetyl CoA thiolase, Acetoacetyl-CoA thiolase, Acetyl-CoA acetyltransferase, mitochondrial, EC:2.3.1.9
计算分子量	45 kDa
观测分子量	38-45 kDa
GenBank蛋白编号	BC010942
基因名称	ACAT1
Gene ID (NCBI)	38
RRID	AB_2220210
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	P24752
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

ACAT1 (acetyl-CoA acetyltransferase), is a mitochondrial enzyme catalyzing the reversible formation or breakdown of acetoacetyl-CoA and plays important roles in ketogenesis and ketolysis. Deficiency of ACAT1 causes an inherited metabolic disorder. Recently ACAT1 has been reported to get involved in cancer progression.

实验方案

Product Specific Protocols
WB protocol for ACAT1 antibody 16215-1-AP	Download protocol
IHC protocol for ACAT1 antibody 16215-1-AP	Download protocol
IF protocol for ACAT1 antibody 16215-1-AP	Download protocol
IP protocol for ACAT1 antibody 16215-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	IF	Adv Mater Supramolecular Hydrogel with Ultra-Rapid Cell-Mediated Network Adaptation for Enhancing Cellular Metabolic Energetics and Tissue Regeneration Authors - Zhuo Li View Article
mouse	WB	Cell Metab TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis. Authors - Dong Huang View Article
human	WB	Cell Res Hepatocellular carcinoma redirects to ketolysis for progression under nutrition deprivation stress. Authors - De Huang View Article
Mouse	WB	J Clin Invest Acetaldehyde dehydrogenase 2 interactions with LDLR and AMPK regulate foam cell formation. Authors - Shanshan Zhong View Article
mouse	WB	Sci Adv Ketogenic diet uncovers differential metabolic plasticity of brain cells Authors - Tim Düking View Article
mouse,human	WB	Phytomedicine Rice bran active peptide (RBAP) inhibited macrophage differentiation to foam cell and atherosclerosis in mice via regulating cholesterol efflux Authors - Jianfei Mu View Article

验证数据展示

WB analysis of MCF-7 using 16215-1-AP

WB analysis using 16215-1-AP

IHC staining of human liver using 16215-1-AP

FC experiment of HeLa using 16215-1-AP

IP experiment of mouse heart using 16215-1-AP

IHC staining of human liver using 16215-1-AP

IHC staining of human colon using 16215-1-AP

IF Staining of HeLa using 16215-1-AP

ACAT1 Polyclonal antibody

ACAT1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Supramolecular Hydrogel with Ultra-Rapid Cell-Mediated Network Adaptation for Enhancing Cellular Metabolic Energetics and Tissue Regeneration

TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis.

Hepatocellular carcinoma redirects to ketolysis for progression under nutrition deprivation stress.

Acetaldehyde dehydrogenase 2 interactions with LDLR and AMPK regulate foam cell formation.

Ketogenic diet uncovers differential metabolic plasticity of brain cells

Rice bran active peptide (RBAP) inhibited macrophage differentiation to foam cell and atherosclerosis in mice via regulating cholesterol efflux